Immunolocalization of Actin in Paramecium Cells
نویسندگان
چکیده
منابع مشابه
Immunolocalization of actin in Paramecium cells.
We have selected a conserved immunogenic region from several actin genes of Paramecium, recently cloned in our laboratory, to prepare antibodies for Western blots and immunolocalization. According to cell fractionation analysis, most actin is structurebound. Immunofluorescence shows signal enriched in the cell cortex, notably around ciliary basal bodies (identified by anti-centrin antibodies), ...
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In many vertebrate nonmuscle cells, the microfilament subunit protein, actin, exists as two isoforms, called beta and gamma, whose sequences differ only in their amino-terminal regions. We have prepared a peptide antibody specifically reactive with the amino-terminal sequence of gamma actin. This antibody reacted with nonmuscle actin as determined by Western blots of SDS gels, and reacted with ...
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Most data available from Paramecium, fewer from Tetrahymena, disclose essentially the same principles of signaling as in metazoans up to man. Microtubules serve for long-range signaling, whereas SNARE proteins, H-ATPase, GTPases and actin provide short-range molecular signals, with Ca as a most efficient, locally and spatially restricted signal particularly for membrane fusion. This is enabled ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2004
ISSN: 0022-1554,1551-5044
DOI: 10.1369/jhc.4a6379.2004